Latest Research

  • CHOROIDEREMIA RESEARCH FOUNDATION HOLDS 2019 INTERNATIONAL CHOROIDEREMIA SYMPOSIUM June 14, 2019

    On June 6-7, the Choroideremia Research Foundation (CRF) hosted its 2019 International Choroideremia Symposium in Philadelphia, Pennsylvania.  With the support of the Center for Advanced Retinal and Ophthalmic Therapeutics at the University of Pennsylvania, CRF developed an agenda geared toward answering key questions raised by ongoing research, and developing consensus opinion on paths forward for the organization and its community.  The Symposium was attended by 20 international experts in CHM from 5 countries in addition to representatives from biotech industry and the patient community to bring multiple perspectives toward the singular goal of developing treatments to end blindness.

    The Symposium was held using an open moderated discussion format, engaging all in attendance to help discuss topics and answer questions using the collective expertise of those in attendance.  The first day focused on pre-clinical topics which could provide better understanding of disease mechanisms and, in corollary, additional avenues toward the development of new therapies.  Much discussion revolved around the underlying mechanisms of retinal cell dysfunction and death in CHM, which like many retinal diseases requires additional investigations.  The group discussed a series of potential therapeutic approaches including the use of stem cells, neuroprotective agents, optogenetics, and reversal of the normal aging process in cells, and how research on these approaches could be supported.  Discussions on day 2 of the Symposium reviewed ongoing natural history studies and clinical trials, bringing together leaders in these respective areas to identify lessons learned from these trials and potential next steps.  Lastly, a robust discussion was held to begin developing a classification system to stage the progression of CHM in patients, as well as the ideal outcome measures to be used in future clinical trials.

    “The 2019 International Choroideremia Symposium was a tremendous success,” says Christopher Moen, Chief Medical Officer of CRF.  “The topics reviewed at the Symposium identified several opportunities for research projects which could drive CHM toward future therapies that could work alongside gene replacement therapy.  We’re excited at the future of CHM research and fortunate to have such tremendous support from our medical and scientific community.”

    About the Choroideremia Research Foundation

    CRF was founded in 2000 as a fundraising and patient advocacy organization to stimulate research on CHM. Since its inception, the CRF has provided over $2 million in research awards and is the largest financial supporter of CHM research worldwide. Research funded by the CRF has led to the development of a CHM animal model, the pre-clinical production of gene therapy vectors currently in clinical trials, and the CRF Biobank which stores tissue and stem cell samples donated by CHM patients.

  • 4DMT and ROCHE Expand Ophthalmology Partnership to Develop and Commercialize Multiple AAV Gene Therapy Products May 2, 2018

    4D Molecular Therapeutics (4DMT), a leader in Therapeutic Vector Evolution for adeno-associated virus (AAV) gene therapy vector discovery and product development, today announced the expansion of its 2016 research agreement with F. Hoffmann-La Roche Ltd and Hoffmann-La Roche Inc. into a broad long-term partnership to develop and commercialize multiple ophthalmology products.

    The expanded agreement will allow each company to leverage its primary strengths to bring highly-optimized gene therapeutics to ophthalmology patients as expeditiously as possible. 4DMT will be responsible for vector discovery and optimization, product design and engineering, pre-clinical and early-stage clinical development, including manufacturing activities, while Roche will conduct pivotal clinical trials and commercialize the new therapeutics globally.

    4DMT’s intravitreally-delivered choroideremia clinical candidate, 4D-110, is the first collaboration program; IND-enabling studies and activities are underway. Additional clinical candidate development programs are underway to treat retinal diseases with high unmet need.

    “Together we elected to expand our partnership after 4DMT completed proprietary intravitreal vector discovery and characterization. We have created clear synergies between 4D’s vector discovery, gene therapy development and manufacturing capabilities and Roche’s expertise in late-stage clinical development and global biologics commercialization. The decision to expand our partnership represents validation of our ophthalmology platform, clinical candidates and team,” said David Kirn MD, CEO and co-founder of 4DMT.

    “This expanded partnership could prove a major catalyst to bring new therapies for blinding retinal disorders. 4D’s gene therapy technology and Roche’s expertise in biologics and should be a powerful combination,” says Jeffrey S. Heier, MD, Co-President and Medical Director, Ophthalmic Consultants of Boston.

    “The expansion of this partnership holds promise for accelerating treatments for our patients with retinal diseases,” says Dr. Stephen Rose, Chief Scientific Officer of Foundation Fighting Blindness, positioned as the largest non-governmental/non-commercial supporter of R&D for inherited retinal degenerations.

    “The prospect of an intravitreally-delivered gene therapy for choroideremia could potentially benefit the whole population of patients with this condition,” explains Ian MacDonald MD, Professor in the Department of Ophthalmology and Visual Sciences, University of Alberta.

    Affecting approximately 200,000 patients in the US and similar numbers in Europe, inherited retinal diseases are, collectively, a major cause of adult and childhood blindness. Mutations in more than 200 genes are known to cause these rare, orphan conditions for which there are currently few approved therapies. More common diseases of the retina affect several million patients in the US and EU.

    About 4D Molecular Therapeutics (4DMT)

    4DMT is focused on the discovery and development of targeted and proprietary AAV gene therapy products for use in patients with severe genetic diseases with high unmet medical need. Our robust discovery platform, termed Therapeutic Vector Evolution, empowers us to create customized gene delivery vehicles to deliver genes specifically to any tissue or organ in the body, by optimal clinical routes of administration, at manageable doses and with resistance to pre-existing antibodies. These proprietary and targeted products may be developed to treat both rare genetic diseases and large market complex diseases. 4DMT is creating a diverse and deep product pipeline through its own internal 4D products, as well as through partnered programs.

    4DMT patient advocacy organization partners in ophthalmology include Foundation Fighting Blindness and Choroideremia Research Foundation.

    About 4DMT’s Therapeutic Vector Evolution

    Gene therapy has shown promise for the treatment of rare diseases, yet current clinical stage gene therapy products are not targeted and are generally based on one of a few AAV vectors that are “naturally-occurring” or “wild-type”, meaning they were found in nature (e.g. as laboratory contaminants or as monkey infections). These first-generation AAV vectors, while generally safe and well-tolerated in patients, do not have targeted or optimized delivery properties and often require aggressive and/or invasive injection at high doses to attempt the desired transduction of target cells in the body. 4DMT is advancing the field of AAV vector technology by deploying principles of evolution and natural selection to create vectors that efficiently and selectively target the desired cells within the diseased human organ via clinically optimal routes of administration, at manageable doses and with resistance to pre-existing antibodies in the population. Our Therapeutic Vector Evolution platform deploys an estimated 100 million unique AAV variants with extensive diversity, from over 35 unique and proprietary 4DMT AAV vector libraries. After defining the Target Product Profile, and the associated Target Vector Profile, 4DMT then applies proprietary methods to identify lead vectors for the specific Target Vector Profile from within our AAV libraries. The result is a customized, novel, and proprietary pharmaceutical-grade product uniquely designed for targeted therapeutic gene delivery and efficacy in humans.

  • Nightstar Announces First-Ever Phase 3 Choroideremia Gene Therapy Trial March 26, 2018

    Nightstar Therapeutics has announced the initiation of the company’s STAR Phase 3 registrational trial to study the safety and efficacy of NSR-REP1 in patients with choroideremia. In data from 32 patients treated with NSR-REP1 across four open-label Phase 1/2 clinical trials, over 90% of treated patients maintained or improved their visual acuity over a one-year follow-up period.

    The STAR trial is expected to enroll approximately 140 patients across 18 clinical sites in the United States, Europe, Canada and South America, of which six sites will be surgical centers. Eligible patients will be randomized into one of three study arms: 56 patients receiving a high-dose of NSR-REP1 in one-eye (1.0 × 10^11 genome particles, or gp); 28 patients receiving a low-dose of NSR-REP1 in one-eye (1.0 × 10^10 gp); and 56 patients receiving no treatment (no-sham, parallel control arm). Patients in the STAR trial are expected to be recruited primarily from the existing Nightstar-sponsored natural history observational study (NIGHT study) in order to accelerate Phase 3 enrollment from this well-characterized patient population. The primary endpoint of the STAR trial is the proportion of patients with an improvement of at least 15 ETDRS letters from baseline in visual acuity at 12 months post-treatment. The primary endpoint will compare patients in the high-dose treatment arm with patients in the control arm.

    “The initiation of this first-ever Phase 3 trial for the treatment of choroideremia is a major milestone for Nightstar and a tremendous step forward for patients otherwise at risk of blindness due to this devastating disease,” said Dave Fellows, chief executive officer of Nightstar. “We are very encouraged by the responses we have seen to-date following treatment with NSR-REP1. This accomplishment demonstrates our team’s ability to successfully advance important gene therapies. We are thankful to our academic and advocacy partners, as well as the many patients who have participated in our studies, all of whom have been instrumental in helping us to achieve this milestone.”

    “The Choroideremia Research Foundation is encouraged by the advancement of this gene therapy and congratulates the Nightstar team for their unrelenting commitment to serving patients,” said Randy Wheelock, chief advisor for research and therapy development for the Choroideremia Research Foundation.

    Dr. Christopher Moen, president of the CRF commented, “Not only is this important for choroideremia patients and their families, but it is another important step toward developing therapies for the many people affected by blinding inherited retinal diseases, of which over 200 have been identified. The CRF is proud of its contributions in helping Nightstar achieve this milestone, including grants for initial research and preclinical studies. We look forward to realizing the full potential NSR-REP1 could have for patients with this challenging condition.”

  • Nightstar Therapeutics raises $75 Million in IPO to fund Pivotal Phase 3 Gene Therapy Study October 1, 2017

    Nightstar Therapeutics held it’s Initial Public Offering on Thursday September 28th, raising $75 Million which will be used to advance the company’s gene therapy treatment for Choroideremia along with other Retinal Degenerative Diseases the company is working on.  The company, which was spun out of Oxford University has completed Phase 1/2 Clinical Trials and is preparing to undertake Pivotal Phase 3 trials for their gene therapy treatment targeting Choroideremia.  The Nightstar treatment involves the use of an adeno-associated viral (AAV) vector to deliver a corrected version of the Gene that causes Choroideremia.  Because gene therapy treatment is aims to replace the defective gene with a corrected version of the gene, the treatment is designed as a one time injection.

    Choroideria Research Foundation President Dr. Christopher Moen and wife Alis Moen were invited to attend the NASDAQ Closing Bell Ceremony by Nightstar CEO David Fellows.  Dr. Moen and the Choroideremia Research Foundation were recognized on stage during the Closing Bell ceremony.  The Choroideremia Research Foundation provided grants in support of the early work performed by Dr. Miguel Seabra which laid the foundation for the development of the Nightstar Therapeutics gene therapy treatment, and has provided support for lead surgeon and Nightstar Co-Founder Dr. Robert MacLaren.

    For more information on Nightstar Therapeutics and their gene therapy development you can visit their website by clicking here.  Nightstar Therapeutics is now being traded on the NASDAQ under the symbol NITE.

  • Spark Announces Phase 3 Data for LCA Trial October 5, 2015

    20150101_inq_spark01-cAn announcement today by Spark Therapeutics brings the world one step closer toward a commercially available gene therapy treatment.  This morning Spark released positive results from the Phase 3 pivotal trial of its lead gene therapy product candidate, SPK-RPE65, for the treatment of RPE65-mediated inherited retinal dystrophies, more commonly known as LCA.

    “We saw substantial restoration of vision in patients who were progressing toward complete blindness,” said Albert M. Maguire, MD, principal investigator in the trial and professor of ophthalmology at the Perelman School of Medicine of the University of Pennsylvania. “The majority of the subjects given SPKRPE65 derived the maximum possible benefit that we could measure on the primary visual function test, and this impressive effect was confirmed by a parallel improvement in retinal sensitivity. If approved, SPK-RPE65 should have a positive, meaningful impact on the lives of patients with this debilitating condition.”

    The phase 3 study evaluated 31 patients, 21 of whom were treated and 10 who represented controls.  Patients treated with SPK-RPE65 were able to navigate through a mobility course more effectively at varying levels of light after treatment was provided.  They also were found to have improved full-field light sensitivity threshold testing, which is a measure of physiologic function of the retina.  In addition, there were no serious adverse events or concerning immune responses observed in the clinical trial.  These results show evidence of success of the gene therapy treatment and

    “These results are the culmination of more than a decade of work of many dedicated individuals to correct the underlying cause of RPE65-mediated blindness through the one-time administration of a gene therapy,” said Jean Bennett, MD, PhD, professor of ophthalmology and director of the Center for Advanced Retinal and Ocular Therapeutics at the Perelman School of Medicine of the University of Pennsylvania. “We are excited about the potential impact that the results will have on the treatment of this and other blinding conditions.”

    Currently, there are no commercially available gene therapy treatments in the United States and only one in the Western world.  These positive results bring Spark one step closer to approval for SPK-RPE65, which would also be the first gene therapy treatment for a retinal degenerative disease.  Successful progress and approval of SPK-RPE65 bodes well for Choroideremia, currently in Phase 1/2 clinical trials by Spark.  With the success and experience from the RPE65 program now established, the Choroideremia community hopes that their disease will follow a similar, if not simpler, path through the clinical trial and regulatory process.

    For more information on Spark Therapeutics, you can navigate to their website at www.sparktx.com.  In addition, patients can contact Spark directly at 800-SPARKTX or patients@sparktx.com for more information.

     

  • Clinical Trials Announced in Miami September 19, 2015

    photo

    The Bascom Palmer Eye Institute at the University of Miami has announced the opening of a clinical trial using gene therapy on Choroideremia patients.  The clinical trial, titled An Open Label Phase 2 Clinical Trial of Retinal Gene Therapy for Choroideremia Using an Adeno-associated Viral Vector (AAV2) Encoding Rab-escort Protein 1 (REP1), was officially posted on the website www.clinicaltrials.gov on September 17th.  Led by Dr. Byron Lam, the phase II clinical trial will treat 6 male patients with a sub-retinal injection of the AAV2-REP1 gene therapy vector in one eye, using the second eye for comparison.  Patients will be followed for a total of 11 visits over 24 months with an additional 3 year follow-up period.  All patients are required to be 18 years or older, have a genetic diagnosis of Choroideremia, and have visible disease in their retina on examination.  The clinical trial team will be evaluating both the safety of the treatment as well as its effectiveness in treating the diseased eye.

    “The Choroideremia Research Foundation is excited to report the announcement of clinical trials for Choroideremia at the Bascom Palmer Eye Institute,” says Christopher Moen, CRF President.  “Bascom Palmer is an internationally recognized leader in ophthalmology care, and we are excited that they and Dr. Lam have chosen to study Choroideremia.  This clinical trial will expand access to gene therapy to individuals with Choroideremia in the United States, and will continue progress toward an approved treatment.  We believe in the potential for a successful gene therapy treatment for Choroideremia, and this study will bring us even closer to that goal.”

    The Bascom Palmer study marks the fourth clinical trial using gene therapy to treat Choroideremia around the globe.  Clinical trials are currently underway at the University of Oxford by Dr. Robert Maclaren, the University of Alberta by Dr. Ian Macdonald, and at Children’s Hospital of Philadelphia and the University of Pennsylvania by Spark Therapeutics. In 2014, Dr. Maclaren reported the preliminary results of the University of Oxford clinical trial in the medical journal The Lancet.  In the six months after treatment with this therapy, the first six patients showed improvement in their vision in dim light and the two patients who had impaired visual acuity at the start of the trial were able to read more lines on the eye chart.

    Enrollment for this study is underway currently.  Individuals who are interested in learning more about the clinical trial, or who are considering enrolling as a patient, can go to the study page on the clinicaltrials.gov website for more details and contact information.

  • Gene Therapy Clinical Trials Begin in Canada May 28, 2015

    Dr. Ian MacDonaldThe University of Alberta, in coordination with NightstaRx, have initiated a clinical trial for patients with Choroideremia using NightstaRx’s gene therapy product AAV2-REP1. The phase 1 trial is an open-label study designed to test the safety and preliminary efficacy of sub-retinal injection of AAV2-REP1. The trial will be performed at the University of Alberta under the supervision of Dr. Ian MacDdonald and will enroll 6 patients with identified CHM mutations. “For many of us, CHMers and researchers, this is an exciting time, one that has been anticipated for many years”, says MacDonald.
    “The Choroideremia Research Foundation is thrilled at the initiation of clinical trials at the University of Alberta,” says Chris Moen, President of the CRF. “Dr. MacDonald has supported the CRF and Choroideremia patients for years, and we are excited to see his team, in conjunction with NightstaRx, bring gene therapy trials to Canada.This is a tremendous opportunity to make gene therapy accessible to patients in Canada who are losing their sight.”
    Pre-clinical development of the AAV2-REP1 vector was performed by Profs. Miguel Seabra and Robert Maclaren with financial support from the Choroideremia Research Foundation. The vector is currently being studied by Prof. Maclaren in a Phase 1/2 clinical trial at the University of Oxford, UK. Results published in The Lancet Medical Journal in January 2014 reported that six months after treatment with this therapy, the first six patients showed improvement in their vision in dim light and the two patients who had impaired visual acuity at the start of the trial were able to read more lines on the eye chart. The Phase 1/2 study is currently ongoing and, according to the NightstaRx website, the company will soon announce its plans for future studies.
    For more information on NighstaRx and its ongoing gene therapy work, please visit www.nightstarx.com.
    To learn more about the University of Alberta clinical trial, you can visit the Choroideremia Gene Therapy at the University of Alberta website at www.chmgenetherapy.ca or to the study page on the clinicaltrials.gov website.

  • Nightstar Receives U.S. and European Orphan Drug Designation for Gene Therapy to Treat Choroideremia March 24, 2015

    PRESS RELEASE:

    London, 11 January 2015 – NightstaRx Ltd (“Nightstar”), the biopharmaceutical company specialising in bringing therapies for retinal dystrophies to patients, has received both U.S. Food and Drug Administration (FDA) and European Medicines Agency (EMA) Orphan Drug Designation for its lead programme, a gene therapy to treat Choroideremia, an X-linked recessive disorder that leads to progressive blindness.


    Orphan Drug Designation, which is intended to facilitate drug development for rare diseases, provides substantial benefits to the sponsor, including regulatory support in development activities such as protocol assistance, reduced fees, tax incentives and several years of market exclusivity for the product upon regulatory approval.

    Click Here to read the full press release

  • SPARK Therapeutics pulls off a $161M IPO January 30, 2015

    jeffrey-d-marrazzoSpark Therapeutics, the Philadelphia firm specializing in gene therapy treatments who just last week announced the start of the United States first clinical trial to treat Choroideremia using it’s gene therapy product SPK-CHM, has gone public today on the NASDAQ bringing in $161 million during it’s Initial Public Offering.  Chief Executive Officer Jeffrey D. Marrazzo and the team at Spark have worked closely with University of Pennsylvania and Dr. Jean Bennett to help bring the SPK-CHM Gene Therapy into phase 1 clinical trials.

    Read the Philadelphia Business Journal Blog about this IPO by clicking here

    Read more about this IPO on Fierce Biotech by clicking here.

  • First U.S. Gene Therapy Clinical Trial to treat Choroideremia initiated in Philadelphia January 20, 2015

    Dr. Jean BennettSpark Therapeutics, a late-stage gene therapy company developing treatments for debilitating, genetic diseases, announced today it has initiated a Phase 1/2 clinical trial for the potential treatment of patients with choroideremia (CHM) utilizing its gene therapy product SPK-CHM.  The Phase 1/2 trial is an open-label, dose-escalating trial designed to assess the safety and preliminary efficacy of sub-retinal administration of SPK-CHM. The Phase 1/2 trial will be conducted at The Children’s Hospital of Philadelphia (CHOP) and the University of Pennsylvania, and has plans to enroll up to 10 patients afflicted with the CHM genetic mutation.

     

    “Spark’s groundbreaking announcement today brings real hope of a cure for blindness caused by choroideremia, and promises to pave the way for treatments of other retinal diseases impacting millions of people around the World,” said Dr. Chris Moen, President of the Choroideremia Research Foundation, the leading advocacy and fundraising organization focused on finding a cure for CHM.  “The Choroideremia Research Foundation is proud to have provided preclinical funding to Jean Bennett, MD, PhD, and her team at the Perelman School of Medicine at the University of Pennsylvania, that has helped bring us to the gene therapy human clinical trials being announced today.”

     

    Dr. Jean Bennett’s preclinical work, which was funded in part by consistent financial support from the Choroideremia Research Foundation (curechm.org), demonstrated the ability of the SPK-CHM gene therapy to restore REP-1 protein production, membrane trafficking and retinal structure.

     

    “Throughout my career’s work developing genetic therapies for inherited retinal dystrophies I have had my target set on a number of different conditions, in particular, choroideremia,” said Dr. Bennett, who is also one of Spark’s scientific co-founders and a scientific advisor on the SPK-RPE65 clinical trials being conducted at CHOP. “The SPK-CHM program, for the first time, creates the potential for patients to use their vision for longer and see more things.”

     

    In addition to evaluating safety, the trial will help define the dose required to achieve stable or improved visual function and identify appropriate endpoints for subsequent clinical trials. With SPK-CHM, Spark is leveraging the experience and technology utilized in the development of its gene therapy for Leber’s congenital amaurosis (LCA), SPK-RPE65, including the same vector, target cells and route of administration, as well as the same manufacturing process.  SPK-RPE65 is currently in a fully-enrolled pivotal Phase 3 clinical trial.

     

    CHM is an X-linked inherited retinal dystrophy which manifests in affected males in childhood as night blindness and a reduction of visual field, followed by progressive constriction of visual fields, ultimately leading to complete blindness.  CHM affects an estimated 1 in 50,000 males in the United States and there is currently no approved treatment for the disease.

     

    For more information on Choroideremia and the Choroideremia Research Foundation (CRF), visit http://www.curechm.org/

     

    For more information on Spark and its pipeline of gene therapy candidates please visit www.sparktx.com/pipeline.

532 thoughts on “Latest Research

  1. Pingback: Cocktail Dresses
  2. Pingback: pe bible results
  3. Pingback: Jumpsuits
  4. Pingback: Foundation
  5. Pingback: pe bible
  6. Pingback: Cosmetics
  7. Pingback: Buy and Sell
  8. Pingback: Teen gifts
  9. Pingback: Web Site
  10. Pingback: VR
  11. Pingback: click for source
  12. Pingback: pickup ??
  13. Pingback: Womens Jackets
  14. Pingback: Continue Reading
  15. Pingback: xxx àíàë
  16. Pingback: Technorotic.com
  17. Pingback: kayak fishing seat
  18. Pingback: Goziyan.com
  19. Pingback: LED PANEL TROUFFER
  20. Pingback: snook fishing
  21. Pingback: Technozan.com
  22. Pingback: tramadol
  23. Pingback: mature dating
  24. Pingback: dating websites
  25. Pingback: Get More Info
  26. Pingback: agen bola
  27. Pingback: obat kuat
  28. Pingback: obat kuat pria
  29. Pingback: cream hn
  30. Pingback: 60ml e juice
  31. Pingback: 60ml eliquid
  32. Pingback: e ejuice
  33. Pingback: 60ml e liquid
  34. Pingback: schedule email
  35. Pingback: bangalore escorts
  36. Pingback: forex
  37. Pingback: best beard oils
  38. Pingback: Cleaner Brooklyn
  39. Pingback: Airbnb Credit
  40. Pingback: online sales pro
  41. Pingback: kapil matka
  42. Pingback: kapil matka
  43. Pingback: Best Recliners
  44. Pingback: iPhone 7
  45. Pingback: core promotion
  46. Pingback: filme stream
  47. Pingback: reserver etc
  48. Pingback: Kid Chronic
  49. Pingback: Barack Obama 2016
  50. Pingback: GLD ETF
  51. Pingback: cars
  52. Pingback: Discover More Here
  53. Pingback: Related Site
  54. Pingback: pillow malaysia
  55. Pingback: gold media
  56. Pingback: Christian Gold
  57. Pingback: online marketing
  58. Pingback: custom pillows
  59. Pingback: MRA
  60. Pingback: pillows customised
  61. Pingback: pillows
  62. Pingback: satta king
  63. Pingback: khun thailand
  64. Pingback: Smart Meters
  65. Pingback: lanetyper
  66. Pingback: manila milk tea
  67. Pingback: lanetyper
  68. Pingback: drake fake love
  69. Pingback: How to link email
  70. Pingback: IRCTC
  71. Pingback: tea toast
  72. Pingback: smart meter uk
  73. Pingback: mongolia ecotravel
  74. Pingback: hiking to Mongolia
  75. Pingback: greek servers
  76. Pingback: trek mongolia
  77. Pingback: botox
  78. Pingback: Danya Feltzin
  79. Pingback: here
  80. Pingback: digital marketing
  81. Pingback: Dominos coupons
  82. Pingback: civil case lawyers
  83. Pingback: Ryan Hampton
  84. Pingback: Safe Pass Galway
  85. Pingback: Paternity testing
  86. Pingback: computer support
  87. Pingback: DNA test
  88. Pingback: Free gmail add-ons
  89. Pingback: uk badges
  90. Pingback: custom patches
  91. Pingback: buy cheap cr2016
  92. Pingback: order dumpster
  93. Pingback: buy cr2016 battery
  94. Pingback: AB 508 Training
  95. Pingback: doggystyle gif
  96. Pingback: pussy-licking gif
  97. Pingback: pyramid necklace
  98. Pingback: 4ft6 Double Bed
  99. Pingback: amoxicillin
  100. Pingback: Contemporary Beds
  101. Pingback: Mattress Reviews
  102. Pingback: Equity Position
  103. Pingback: tantric massage
  104. Pingback: Click Here
  105. Pingback: Manual testing
  106. Pingback: Getting a mortgage
  107. Pingback: london tantric
  108. Pingback: colacoaster
  109. Pingback: sensual massage
  110. Pingback: sountrack movie
  111. Pingback: weight loss pill
  112. Pingback: Surf exchange
  113. Pingback: weight loss plan
  114. Pingback: free music
  115. Pingback: hair dryer brush
  116. Pingback: Chargepoint CPF25
  117. Pingback: Dryer fires
  118. Pingback: books about time
  119. Pingback: Business Process
  120. Pingback: aromasuperstore
  121. Pingback: Jan Karpiel
  122. Pingback: towing
  123. Pingback: Karpiel Bikes
  124. Pingback: casinoswing
  125. Pingback: bonus casino
  126. Pingback: Loyalty App
  127. Pingback: Loyalty App
  128. Pingback: casinos online nj
  129. Pingback: Driving school
  130. Pingback: miami tummy tuck
  131. Pingback: Grant Money Search
  132. Pingback: adult dating
  133. Pingback: Grant Writhing
  134. Pingback: advise
  135. Pingback: cottage signs
  136. Pingback: Dryer lint fire
  137. Pingback: custom plaques
  138. Pingback: golf signs
  139. Pingback: lake appliance
  140. Pingback: Mr. SEO Hero
  141. Pingback: facelift
  142. Pingback: Mr. SEO Hero
  143. Pingback: Senior Photos
  144. Pingback: here
  145. Pingback: home address signs
  146. Pingback: High School Photos
  147. Pingback: exterior golf sign
  148. Pingback: Yearbook Photos
  149. Pingback: Yearbook Photos
  150. Pingback: tasks
  151. Pingback: Vietnam vacations
  152. Pingback: ZACHARY
  153. Pingback: RILEY
  154. Pingback: HandyPro Handyman
  155. Pingback: TOMMY
  156. Pingback: Satta Matka
  157. Pingback: Best Photography
  158. Pingback: clean dryer lint
  159. Pingback: click here
  160. Pingback: wood craft 
  161. Pingback: 350 words
  162. Pingback: key to growth
  163. Pingback: SEO
  164. Pingback: uniforms
  165. Pingback: Website Design
  166. Pingback: cosplay costume
  167. Pingback: Escort Frankfurt
  168. Pingback: Escort Frankfurt
  169. Pingback: polyurethane
  170. Pingback: SCR888 Malaysia
  171. Pingback: forex
  172. Pingback: Online SCR888
  173. Pingback: forex
  174. Pingback: SCR888
  175. Pingback: Online SCR888
  176. Pingback: Alexander McQueen
  177. Pingback: PS4 vs xbox one
  178. Pingback: SecurityNewsOnline
  179. Pingback: SecurityNewsOnline
  180. Pingback: SecurityNewsOnline
  181. Pingback: check it out
  182. Pingback: go right here
  183. Pingback: you could try here
  184. Pingback: Read Full Article
  185. Pingback: go to my blog
  186. Pingback: online deals
  187. Pingback: Ivans Clothing
  188. Pingback: Online Services
  189. Pingback: EXOTIC DANCERS
  190. Pingback: FEMALE STRIPPERS
  191. Pingback: ORLANDO STRIPPERS
  192. Pingback: OpenLink
  193. Pingback: OpenLink
  194. Pingback: OpenLink
  195. Pingback: OpenLink
  196. Pingback: OpenLink
  197. Pingback: kepsar
  198. Pingback: keps
  199. Pingback: howitworks
  200. Pingback: merchants
  201. Pingback: world-animal-day
  202. Pingback: bathroom sink tops
  203. Pingback: world-homeless-day
  204. Pingback: farmhouse sink
  205. Pingback: composite sinks
  206. Pingback: laundry room sink
  207. Pingback: waterfall faucets
  208. Pingback: farm sink
  209. Pingback: granite sinks
  210. Pingback: composite sinks
  211. Pingback: Shraddha Kapoor
  212. Pingback: Redfoo
  213. Pingback: composite sing
  214. Pingback: glass vessel sinks
  215. Pingback: Sandra Dee
  216. Pingback: drain assembly

Comments are closed.